Posted by
Savannah Momo on Tuesday, December 01, 2009 10:38:04 PM
Pseudomonas putida ZWL73 separated from
4-Nitrophenyl Chloroformate contaminated soil, can be 4CNB as the sole carbon and nitrogen source of
growth, and the release of chloride ions from the substrate and ammonium ion.
Degradation of bacteria by enzymatic analysis of cell extracts to detect pairs
of 4CNB nitro-reductase activity and speculated that the open-loop substrate 2 -
amino -5 - chlorophenol (2A5CP) ring-opening dioxygenase activity, combined with
growth substrate range of factors (such as the product of a full restoration can
not be used for p-chloroaniline growth) and by GC-MS to detect pairs of chlorine
sub-nitrobenzene in the medium, the generation and conversion results suggest
that ZWL73 through some means of restoring degraded 4CNB.
Plasmid elimination
and conjugative transfer showed degrading bacteria has the 100kb plasmid
involved in the
4-Nitrophenyl Chloroformate GC 96% degradation, as evidenced from: plasmid elimination loss of
wild-type bacteria possess 2A5CP nitro reductase and ring-opening dioxygenase
activity; plasmid After the elimination of strains can not grow on the substrate
medium, while the plasmid transfer into the recipient bacteria after the latter
obtained a 4CNB the ability to grow on.
ZWL73 is the first one identified
4-Nitrophenyl Chloroformate Titration 98%
the degradation pathway encoded in plasmid strains. By constructing plasmid DNA
library was screened with a right 2A5CP activity of clones, obtained by
subcloning a 4.9kb EcoRI fragment, sequenced fragment of the sequence analysis
showed that the two genes encoding the inter-bit cnbCaCb were the open-loop
pairs plus oxygenase β and α subunit; over-expressed in E. coli proteins show
...